• Rapid microbiology
• Cell proliferation/Cytotoxicity
• Hygiene control
• Enzymes & metabolites
• Reporter gene studies
• Immunoassays
• Kinase Kit for HTS & research

Enzymes and metabolites

	Enzymes and metabolites
Reaction is possible to couple to ATP formation or degradation, e.g kinases and their substrate or mitochondrial ATP production No immediately obvious reaction can be coupled to ATP formation or degradation e.g pyrosequencing or measurement of DNA polymera se
144-041 ATP Kit SL  Consult BioThema for suggestions

Applications include: Lipolysis e.g. in obesity research Oxidative phosphorylation e.g. in inborn metabolic diseases Creatine kinase e.g. in acute myocardial infarction ATP/ADP/AMP ratios in apoptosis	ATP/phosphocreatine in muscle metabolism studies Glucose measurement using hexokinase reaction Continuous monitoring of cell lysis Continuous monitoring of platelet aggregation

Principles:

ATP Kit SL contains the stable light emitting ATP Reagent SL. With this reagent the luciferase reaction consumes <1%/min of the ATP concentration. Adding ATP Reagent SL to a sample, in which ATP is formed or degraded, makes it possible to continuously monitor the ATP level by measuring the intensity of the emitted light. At the end of the assay a known amount of ATP is added to the reaction mixture for calibration and to calculate the formation or degradation of ATP in moles. Kinetic as well as end-point assays can be performed. Enzymes as well as metabolites can be assayed.