Kit selection guide

Immunoassays

Biotinylated luciferase coupled to an antibody via streptavidin

ATP producing enzyme coupled to an antibody

484-001
Luciferase Assay Kit SBLA-001 Streptavidin-biotinylated luciferase


Applications include: Bacterial surface antigen studies Receptor binding studies Antigen and antibody quantification

Principles:

The genetically fused biotinylated recombinant thermostable luciferase can be linked via streptavidin to any biotinylated primary or secondary antibody. After washing and addition of luciferin and ATP, luminescence output can be immediately recorded in a luminometer. This is a highly sensitive assay with a detection limit of 10^-20 mol luciferase.

An even higher sensitivity can be obtained by using biotinylated acetate kinase (not commercially available). ADP + acetyl phosphate -----> acetate + ATP. Bioluminescent detection of the ATP by the firefly luciferase enzyme can be used to detect the marker enzyme at a detection limit of 10^-21 mol.

News

18/09/2009

Can you say it's clean with confidence?

The new and improved ATP Surface Hygiene Monitoring System, Lumitester PD-20, is now available from Kikkoman. Its small size and light weight, ease of use and rapid results makes it suitable for cleanliness control and hygiene training in a range of industrial settings and health care environments. Based on the unique detection of ATP plus AMP, its simple swab and measure method using the new LuciPac Pen swab, makes it possible to detect invisible contamination anywhere and anytime in only 10 seconds with super sensitivity. The easy to read display gives a clear reading of the level of contamination and results can be downloaded to a PC.

01/12/2008

Kinase kit for HTS applications in Drug Development

Our new kit uses direct measurement of ATP degradation rate to distinguish hits from non-hits, in drug development. The methodology is flexible and can easily be adapted to various HTS strategies.